[INTERNSHIP PROPOSALS for 2017/2018]
PCR Fidelity calculator (beta)
ThermoFisher has a PCR Calculator, which is basic. This just calculates ~ [1-l x numcycles x u] (l, fragment length; u, error rate), which is nothing else than the probality for a molecule of length l x numcycles to have no errors. Thus, it assumes 100% efficiency and ignores that some molecules are not replicated and contribute to the overall number of molecules without errors. Here, I use a more rigourous approach (a good article can be found here ). Just enter the parameters below and submit the code (using the Opencpu cloud server; this might takes a few seconds to get the result).
|Enzyme||Error Rate||Fidelity rel. Taq|